Abstract
Iron, zinc, manganese, copper are required for seed maturation and germination. Metal chelators are also required for metal transport during seed maturation and germination of plant. Mugineic acid family phytosiderophores are metal chelators biosynthesized in graminaceous plants, and NA, a chelator of metals, is ubiquitously present in higher plants. Deoxymugineic acid (DMA) is found in rice plant and is produced from NA in two steps catalyzed by nicotianamine aminotransferase and DMA synthase. We investigated the gene expressions in inflorescences and seeds of rice plant by microarray analysis. Transport of metals, NA, and DMA was investigated in inflorescences and seeds of rice using radioisotope. The concentrations of metals, NA and DMA were determined in mature seeds. The results indicated that NA and MAs play a critical role in metal translocation to seeds. In this study, we performed metabolome analysis during seed development of rice seeds using CE/MS to illuminate the relationship between seed maturation and compounds. The roles of organic acids and aimino acids during seed maturation will be discussed.
