Abstract
Tomato is a one of the model plants for Solanaceae species, and is also a benefit crop material for scientists who want to characterize its unique feature such as fruit development. As a part of National BioResourse Project (NBRP) launched in Japan, a total of 10,793 M1 mutagenesis lines of dwarf variety Micro-Tom, consisting of 4,371 and 6,422 lines were generated by EMS-mutagenesis and gamma-ray irradiation, respectively. The M3 seeds we have completed propagating are now available for scientists upon request to NBRP (http://tomato.nbrp.jp/indexEn.html). From our mutagenesis lines, a number of different varieties of mutants defective in organ development have been isolated, categorized based on the plant ontology, and registered in mutant database called TOMATOMA. These mutant recourses are now available upon request through TOMATOMA.
In addition, we constructed the Micro-Tom BAC library consisting of 54,000 clones with an average insert size of 110 kb, covering about 5 times of the haploid genome (930Mb). The 10,8000 genome BAC end sequencing has launched for developing DNA markers, and the determined sequencing information will be available in public.
