Abstract
We have sequenced full-length cDNAs obtained from tomato, Solanum lycopersicum cv Micro-Tom, and opened sequence information in March 2009 at KaFTom database (http://www.pgb.kazusa.or.jp/kaftom/). Here we report a large-scale analysis of this tomato full-length cDNA (FLcDNA) collection. Sequence analysis of UTRs demonstrated that tomato has longer 5'- and 3'-UTRs than most other plants but rice. A comparison of FLcDNAs with genes of sixteen plants facilitated the identification of tomato genes that are not found in other plants. These tomato-specific genes appeared to be enriched with domains associated with DNA- and RNA-binding functions. Mapping of the FLcDNAs onto tomato genome sequences demonstrated that introns of tomato genes were longer than those of Arabidopsis and rice. According to a comparison of exon sequences between the FLcDNAs and the tomato genome sequences, the frequency of single-nucleotide polymorphisms between Micro-Tom and the genome-sequencing cultivar (Heinz 1706) was estimated to be 0.078 %. This work was supported by National Bioresource Project, Genome program, Enhancing tomato resources by sequencing Micro-Tom full-length cDNA (2008, MEXT, Japan).
