Abstract
To develop gene resources related to UVB tolerance of rice, UVB resistant rice cultivar Sasanishiki was irradiated with carbon ion beam (320 MeV: 12C6+, 80 Gy). In this study, we focused two isolated lines, UVB resistant mutant UVTSa-319 and UVB sensitive mutant UVSSa-1. These mutants have similar levels of the amount of UV-absorbing compounds and the repair activities of cyclobutane pyrimidine dimers and (6-4) photoproducts which have been thought as factors related to UVB tolerance to Sasanishiki. We performed Array CGH to estimate mutated region. As a result, we found that approximate 40 kbp on chromosome 7 containing 2 genes (AK111251, AK071492) which have unknown function was deleted in UVTSa-319. In UVSSa-1 mutant, the region on chromosome 7 containing 2 genes (AK073834, AK121807) was deleted. Next, to deduce the relationships between deleted genes and UVB tolerant mechanism in these mutants, microarray analyses were performed using RNAs of Sasanishiki and these mutants. In this meeting, we discuss about these functions of deleted genes and relationships of UVB tolerance or sensitivity.
