Abstract
Our previous studies indicated that OsRac1, a small GTP-binding protein, functions as a key regulator in rice innate immunity. The aim of our study is to reveal the defense signaling pathway in rice by applying the proteome analysis. We isolated OsRac1 interacting proteins by affinity chromatography and identified by using mass spectrometry. They included RACK1, a scaffolding protein, Hsp70 and Hsp90, and Sti1 which is a cochaperone for Hsp70/Hsp90 complex. Also, we identified NB-LRR proteins, which are a typical type of resistance proteins for plant innate immunity. We found that these proteins play a role in ROS production for defense signaling and defensome maintenance by stabilization of OsRac1. We named this protein complex Defensome.
We will show the data obtained from proteomic analysis and discuss the mechanism and the role of defensome in rice innate immunity.
