Abstract
Nucleus is a highly dynamic organelle and has heterogeneous structure, which is composed of double layered nuclear membrane, nuclear pore and nuclear matrix. Because nuclear architecture provides a physical framework for the genetic activities, it is important to know the mechanisms in which structure of the nucleus is built. To reveal the molecular mechanisms of nuclear biogenesis, we did two comprehensive proteomic analyses of Arabidopsis.
Highly purified nuclei were prepared from cultured cells by using concentrated sucrose buffer. More than 1000 proteins were identified with a mass spectrometer (LTQ-Orbitrap XL). Among them, we found novel nuclear membrane proteins, which are not found in animal and yeast cells.
Nuclear pore complex is a large multi protein complex and is composed of more than 30 types of nucleoporins. To clarify the molecular components of nuclear pore complex, we established high-throughput analytical method for protein complexes. By using this method, we identified plant-specific nucleoporins, which might be involved in various steps of plant development.
