Abstract
Effects of light on expression of one of the glycolytic enzymes, fructose-1.6-bisphosphate aldolase (fbaA) were investigated in Synechocystis sp. PCC6803. The fbaA mRNA was induced by light pulse of 5 min in the presence of glucose, while glucose addition in the dark was not effective for induction of fbaA. Therefore, both light and glucose were required for the full induction of fbaA transcription. The induction of fbaA gene expression by light pulse and glucose were inhibited by deletion of gene encoding one of the response regulators, sll1330, suggesting that sll1330 is involved in regulation of fbaA. Investigation of the effects of disruption of the genes encoding other regulatory genes indicated that there was histidine kinase whose disruption promotes fbaA expression in the absence of pulse light under glucose. These results suggest that gene expression of glycolytic enzymes are controlled through complex signal pathways interacting to each other.
