Abstract
To reveal the defense mechanisms of Pinus thunbergii with the pine wood nematode (PWN), Bursaphelenchus xylophilus infection, we have conducted the transcriptome analysis to the PWN-infected resistant and susceptible clone. In previous studies, there has been little approach from the view of transcriptional level, though the difference of defense response between resistant and susceptible clones has been indicated by anatomical and metabolically approach. Kusumoto et al (2010) reported that the phenol accumulation in xylem parenchyma cell of the infected resistant clone was more apparent than that of susceptible clone, and the collapsed ray parenchyma cell in resistant clone was littler than that of susceptible. In this study, we conducted the suppression subtractive hybridization (SSH) analysis using ESTs of the PWN inoculated resistant and susceptible clones. For the time course sampling scheme, it was designed at 1, 3, 7, 14 day post-inoculation based on the previously reported phenotypic data. In this report, we present a characterization of the difference of defense response between resistance and susceptibility in P. thunbergii with PWN infection.
