Abstract
The trans-Golgi network (TGN) is known as a major sorting organelle acting in the secretory pathway. Recent work has suggested that the TGN is also involved in the endocytic pathway in plant cells. To examine how the TGN participates in the endocytosis, it is necessary to visualize the endocytic route in plant cells. Using a Leu-rich repeat transmembrane receptor kinase, FLAGELLIN SENSITIVE2(FLS2) as an endocytosed cargo, we have succeeded in visualization of a process of endocytosis in Nicotiana benthamiana. In order to investigate the proteins that regulate endocytosis on the TGN, we focused on Rab GTPases, small GTPases regulating targeting and/or tethering of transport vesicles. Rab11 is the first target because it has been localized on the TGN. We present how Rab11 mediates endocytic transport on the TGN.
