Abstract
The Golgi apparatus plays an essential role in protein sorting and transport in eukaryotic cells. In animal and plant cells, the Golgi apparatus consists of several flattened membrane sacs called cisternae, which form a stack. However, little is known about the mechanism how this stacked structure is formed and maintained. In vertebrate cells, Golgi stacks are centralized by microtubules and fuse together to form a complex ribbon-like structure. In contrast, individual Golgi stacks are distributed widely in the cytoplasm and move along actin filaments in plant cells. Therefore, plant cells are exceedingly suitable for detailed investigation of the stacked structure. To examine the dynamics of each cisterna, we have established tobacco BY-2 cell lines expressing cis-Golgi and trans-Golgi markers tagged by different fluorescent proteins. The observation revealed that cis-Golgi cisternae and trans-Golgi cisternae behave differently after Brefeldin A (BFA) treatment. We also observed the re-generation of the Golgi apparatus whose stacked structure had been disrupted by BFA treatment. In this meeting, we will discuss the mechanism of the biogenesis of plant Golgi apparatus.
