Abstract
Plants sense balance of carbon (C) and nitrogen (N) metabolites to regulate metabolism and development, called C/N response. To clarify the mechanisms of C/N response, we identified a novel C/N response regulator ATL31, an ubiquitin ligase. Ubiquitin ligase recognizes specific proteins for degradation by ubiquitin-proteasome system. Previous studies demonstrated that the ATL31 is involved in C/N response to degrade the polyubiquitinated protein. To identify the target protein of ATL31, we screened interaction proteins by using immunoprecipitation and MS analysis. Characterization of the identified proteins will be reported.
