Abstract
The balance between carbon (C) and nitrogen (N) availability is an important determinant for different phases of plant growth. However detailed mechanisms regulating C/N response are not well understood. We previously revealed a novel ubiquitin ligase, ATL31, which functions in the C/N response in Arabidopsis thaliana (Sato et al., Plant J, 2009, 60:852-864). In addition, proteomics study with MS analysis demonstrated that 14-3-3 proteins were associated with ATL31 and it was shown that ATL31 has ligase activity against 14-3-3 proteins for direct ubiquitination. Now we are further evaluating the physiological function of 14-3-3 proteins in C/N response regulated by ATL31. The details of biochemical and phenotype analysis of atl31 and 14-3-3 mutants will be reported.
