Plant and Cell Physiology Supplement
Abstract of the Annual Meeting of JSPP 2011
Conference information

Three-dimensional Analysis of Brassica rapa Papilla Cells using Ultra-high Voltage Electron Microscopy
*Megumi IwanoRina NagaiNaoko KajimuraTetsuyuki EntaniAkira IsogaiSeiji Takayama
Author information
CONFERENCE PROCEEDINGS FREE ACCESS

Pages 0359

Details
Abstract
The self-incompatibility (SI) system of the plant species Brassica is controlled by the S-locus, which contains SRK and SP11. SP11 binding to SRK induces SRK autophosphorylation and initiates a signaling cascade leading to the rejection of self-pollen. However, the mechanism controlling hydration and germination arrest during self-pollination is unclear. We observed the concentration of actin bundles at the cross-pollen attachment site and actin reorganization and likely depolymerization at self-pollen attachment site by cytological staining and monitoring transiently expressed GFP-mTalin. The actin-depolymerizing drug cytochalasin D significantly inhibited pollen hydration and germination during cross-pollination. Additionally, UHV-EM tomography revealed the close association of the actin cytoskeleton with an apical vacuole network. Self-pollination disrupted the vacuole network while cross-pollination led to vacuolar rearrangements toward the site of pollen attachment. Taken together, our data suggest that self- and cross-pollination differentially affect the dynamics of actin cytoskeleton leading to changes in vacuolar structure associated with hydration and germination.
Content from these authors
© 2011 by The Japanese Society of Plant Physiologists
Previous article Next article
feedback
Top