Abstract
The roles of xyloglucan in the poplar xylem are being known from the studying poplar overexpressing xyloglucanase, AaXEG2. The specific degradation of xyloglucan in the poplar was confirmed by methylation analysis and immunofluorescence labeling. The fluorescence level owing to CCRC-M1 antibody against xyloglucan were markedly lower in the transgenic xylem than that in the wild type. It was confirmed that the transgenic trees contained less wall-bound xyloglucan than the wild type trees. Since the cell wall modification caused the enhancement of saccharification, an intercalation between cellulose microfibrils and xyloglucans could be one of the resistance factors for cellulose degradation. In this study, localization of xyloglucan could be discussed with saccharification level in the xylem derived from the transgenic trees.
