Abstract
Rice plants show a variety of defense responses upon pathogen attack. To understand the responses of rice plants during the infection by pathogen, we analyzed the gene expression in rice leaf cells infected with M. oryzae using laser microdissection method and rice 44k oligo-DNA array. We used two isogenic rice cv. Nipponbare, NB(Pia) and NB(++), incompatible and compatible with M. oryzae strain, P91-B15, respectively. Hierarchical clustering of microarray results revealed that most remarkable differences in gene expression profiles between incompatible and compatible interaction were observed at 12-24 h post inoculation, in contrast to our previous result where gene expression profiles in whole intact leaves in incompatible interaction were distinct from those in compatible interaction only after 3 days post inoculation. These results suggest that gene expression in rice leaf cells during infection by M. oryzae are regulated spatially, as well as temporally.
