Abstract
Approximately 21,000 of the rice-FOX Arabidopsis lines have been screened for disease resistance against a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000, and a fungal pathogen Colletotrichum higginsianum. 18 lines show high resistance to both pathogens and 6 genes have been shown to confer resistance to both pathogens by retransformation.
Arabidopsis plants overexpressing these genes that were inoculated with C. higginsianum were analyzed by qPCR using PR1 and PDF1.2 as marker genes for SA- and ET/JA- pathways, respectively, and they showed an upregulated ET/JA pathway. One of the 6 genes, BROAD-SPECTRUM RESISTANCE1 (BSR1) encoding a BIK1 like receptor-like cytoplasmic kinase, enabled resistance to both bacterial Xanthomonas oryza and fungal Magnaporthe grisea when overexpressed in rice. We will report on the molecular mechanism of disease resistance against Magnaporthe grisea in BSR1:OX rice by microarray analyses.
