Abstract
It is estimated that nitric oxide is produced and has functions during defense responses against pathogens in plant cells and has a function in signal transduction. S-nirtosylated is one of the post-translational protein modification which is caused by nitric oxide and regulates the protein functions. The identification of S-nirtosylated proteins during plant defense responses is important for elucidation of signal transduction mechanisms mediated by nitric oxide. In this research, we detected S-nirtosylated proteins in GSNO treated potato (Solanum tuberosum) leaf and tuber extract by using biotin switch method. S-nirtosylated proteins were purified and analyzed using nano liquid chromatography in combination with mass spectrometry. We identified proteins from tuber and leaves that represent candidates for S-nitrosylation, including many redox-related proteins.
