Abstract
Chitin is a major MAMP (Microbe-Associated Molecular Pattern) for various fungi and its fragments, chitin oligosaccharides, are known to induce various defense responses in plant cells. Our recent study showed that two types of receptor, CEBiP1) and OsCERK12), play an important role for chitin elicitor signaling in rice. Yeast two-hybrid analyses suggested that CEBiP and OsCERK1 have an ability to form the receptor complex(es) as a hetero- or homo-oligomer. However, Blue Native PAGE and chemical-cross linking experiments indicated that these two molecules are separated from each other, or very weakly associated, in the plasma membrane. To investigate the possibility of the CEBiP-OsCERK1 receptor complex formation in rice cells, we applied immunoprecipitation analysis using the membrane preparation from the rice myc-tagged OsCERK1 cells and found both receptors are directly formed the receptor complex under the chitin elicitor treatment. Recently, we also found that CEBiP is a GPI-anchor typed receptor and is presence in lipid microdomain of rice plasma membrane. Further analyses are in progress.
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2) Shimizu et al, Plant J., 64, 204 ('10).
