Abstract
We had identified brassinosteroid biosynthesis inhibitor Brz insensitive mutant bil1 from EMS-mutation lines, and bil5 from fast neutron-mutation lines. To identify additional components of the brassinosteroid signaling, trial to screen new mutants as Brz-insensitive-long hypocotyls in the dark was applied to Arabidopsis activation-tag lines. We screened Arabidopsis activation-tag lines and isolated bil2 that showed long hypocotyls on the medium containing Brz in the dark. Light-grown bil2 mutants had long petiole phenotype similar to wild-type plants treated with BR or BRI1-OX mutants. These phenotypes suggested that bil2 mutants enhanced the BR signaling. Identification of over expression the bil2 candidate gene is progress.
We are starting to screen new bpg (Brz-insensitive pale green) mutant that showed pale green leaves on the Brz medium in the light. In this study, we used FOX (Full-length cDNA over expresser gene) lines, which were over expression of a normalized Arabidopsis full-length cDNA library.
