Abstract
There is a substantial degree of genetic variation among Arabidopsis wild-type accessions. It is known that accessions vary in many phenotypes, such as seed dormancy and flowering time. However, little is known about variation of endogenous phytohormone levels among accessions. In this study, we performed semi-comprehensive hormone analysis in dry seed in over 150 accessions. In this hormone analysis, endogenous levels of abscisic acid (ABA), indole-3-acetic acid, jasmonic acid, isoleucine-conjugated JA, and salycilic acid were determined. Although any hormones did not show correlation with seed dormancy, there were significant variations in hormone levels among accessions. For example, ABA levels ranged from 47.0 to 506.3 ng/g fresh weight. To identify genetic factors that determine seed hormone levels and seed dormancy, we performed QTL analysis using a hyper-ABA accumulating accession. Several QTLs associating with hormone levels and seed dormancy have been identified so far.
