Abstract
Here, we report identification of selenium resistance gene by QTL analysis using Arabidopsis RILs obtained from Col-0 (selenite resistance) and Ws-2 (selenite sensitive). Our detailed QTL analysis indicates that selenium resistance gene locates between G3883-1.4 and 1H1L-1.4 markers on chromosome 4. Although 199 genes exist at this region, we could not find any candidate gene by gene annotation. Therefore, we narrow down selenium resistance gene from EST database. That is we selected genes that expression is only identified in Col-0. By using this criterion, we found four genes. PCR analysis showed that a genomic deletion, containing At4G19780 and At4G19790 genes, was found only in Ws-2. Furthermore, replacement of promoter region 650bp upstream of At4G19770 gene was also identified in Ws-2. Thus, we carried out gene expression analysis of these genes. The At4G19780 expressed only in Col-0 shoots, while we could not detect all other gene expression in both accessions.
