Abstract
Most terrestrial higher plants usually retard their growth and sometimes die under salt stress. Since soil salinity becomes a cause of low agricultural production in many places, it is crucial to analyze a mechanism of salt tolerant responses of plans.
In the present study, we aimed to elucidate the salt tolerant mechanism of the mangrove plant (Bruguiera sexangula) , which can grow under high salt conditions at the cellular and molecular levels.
Using suspension-cultured cells of Bruguiera sexangula, we observed changes in the vacuolar structure and measured intracellular ion concentrations under salt treatment. The vacuolar volume increased, while ion concentrations decreased after long treatments of high salt. To clarify the molecular mechanism of ion transport, genes of the plasma membrane Na+/H+ antipoter (BsSOS1) and the vacuolar Na+/H+ antipoters (BsNHX2, BsNHX14) were closed and their expression levels under different salt concentrations were measured with the quantitative real-time PCR.
