Abstract
When an intense femtosecond laser pulse is focused into biological tissue or cells through an objective lens, local disruption and/or explosion is induced at the laser focal point with an effective multiphoton absorption. Especially, since the time duration of the femtosecond laser pulse is extremely shorter than the time scale to convert from the light energy to the heat energy, the disruption and explosion are initiated in the time scale before the heat conversion. Namely, the local disruption and explosion with less heating are realized by the femtosecond laser irradiation. Previously, we have developed manipulation and stimulation techniques for single animal cells by utilizing these phenomena. Here I present our new trial to apply these techniques to plant cells or tissue. We have succeeded in function inhabitation of a plant by drilling a part of the tissue and single cell dissection and isolation by scanning the laser on the tissue. Furthermore, we are proposing techniques to stimulate a plant cell mechanically by the local explosion and to introduce outside molecules into the cell.
