Chondroitin sulfate chain of urinary trypsin inhibitor plays an important role in anti-inflammatory effects

抄録

Aim: This study aimed to investigate the role of the chondroitin sulfate (ChS) chain in the anti-inflammatory effect of urinary trypsin inhibitor (UTI), identify ChS structures that enhance the anti-inflammatory activity of UTI, and develop a more effective means of treatment for preterm birth.

Methods: ChS chain-remodeled UTIs were prepared by the hydrolysis and/or transglycosylation reaction of testicular hyaluronidase. Uterine cervical fibroblasts were treated with native or ChS chain-remodeled UTIs and incubated with lipopolysaccharide to induce inflammation. To assess anti-inflammatory effects, interleukin-8 (IL-8) concentrations in culture supernatants were measured by enzyme-linked immunosorbent assay.

Results: Compared with controls without UTI, Ch6S-transferred UTI at the nonreducing terminus significantly suppressed IL-8 release. Other UTIs showed limited suppression.

Conclusion: The Ch6S chain at the nonreducing terminus of UTI enhances the anti-inflammatory activity of UTI. This finding could lead to the development of innovative treatments for preterm birth.