Hypertension Research in Pregnancy
Online ISSN : 2187-9931
Print ISSN : 2187-5987
ISSN-L : 2187-5987
Original Article
Development of new therapeutic agents for preterm birth by glycosaminoglycan chain remodeling of urinary trypsin inhibitor
Seigo Tanaka Ikuko KakizakiKanji TanakaTomoe KodamaAsami Ito-FukuyamaShinichiro SutoRyoki TakahashiYoshihito Yokoyama
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2019 Volume 7 Issue 1 Pages 27-35

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Abstract

Aim: This study aimed to examine the effects of the glycosaminoglycan (GAG) chain in urinary trypsin inhibitor (UTI) on uterine cervical fibroblasts (UCFs) and to apply the findings to the development of more effective therapeutic drugs for the management of preterm birth.

Methods: We prepared GAG chain-remodeled UTIs by hydrolysis and/or transglycosylation by testicular hyaluronidase. These UTIs were added to UCFs obtained from gynecology operations, and the effects of UTIs on the release of IL-8, IL-6, MMP-8, and MMP-9 were examined.

Results: UTIs that were not hydrolyzed tended to reduce IL-8 release more strongly than GAG chain-hydrolyzed UTIs. IL-6 was not affected by GAG chain hydrolysis of UTIs. GAG chain-hydrolyzed UTIs tended to reduce MMP-8 and MMP-9 release more strongly than non-hydrolyzed UTIs.

Conclusions: Our findings suggest that the GAG chain of UTI might reduce hyaluronan during cervical ripening by reducing IL-8 release and has opposite effects on reducing MMP-8 and MMP-9 release related to collagen degradation. This insight may be helpful in the development of more effective therapeutic drugs for the management of preterm birth.

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© 2019 Japan Society for the Study of Hypertension in Pregnancy
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