Abstract
Considering sustainable agriculture, nitrogen-fixing bacteria and arbuscular mycorrhizal fungi (AMF), which are symbiotic microorganisms to increase nutrient absorption by crops, are worthy of attention as alternatives to chemical fertilizers. Compared to conventional cultivation, natural farming, which does not use fertilizer, increases the involvement of symbiotic microorganisms in crops, and it has been found that certain groups of microorganisms are particularly involved in crop roots. It was considered that investigating the functions of these microorganisms individually in the future will contribute to sustainable agriculture from the perspective of reducing the environmental impact.