Abstract
Surgeries, to sites with impaired wound healing capability, often face difficulties. The application of regenerative medicine may provide a breakthrough in dealing with the challenge. The authors have reported a simple, low-cost technique to concentrate bone marrow cells and platelets. The product is called "bone marrow-derived platelet-rich plasma : bm-PRP". When surgeries were performed at sites with impaired wound healing (caused by irradiation, chronic ischemia, long-term administration of steroids), bm-PRP was injected into wound beds and edges. In bm-PRP injected, nucleated cells were concentrated approximately seven-fold to bone marrow aspirate and platelets were three-folds. An aberage of 13.5 × 107 nucleated cells were transplanted per surgery. Wound closure was obtained within three weeks in all patients. No adverse events were encountered in bone marrow harvest or in injection of bm-PRP. Without large-scale facilities, bm-PRP can be prepared in a short period of time at a relatively low cost. Through our experience, we propose supplementation of bm-PRP in performing surgery at sites with impaired wound healing.
