Abstract
Artificial dermis enables the wound coverage to induce thinner, pliable and more favorable reconstruction. Simultaneous use with growth factor and cells are leading to better quality of artificial wound healing. In intractable and chronic radiation injured wounds, autologous adipose-derived stem (regenerative) cells successfully heal the wound for more than 2 years, which previously treated with artificial dermis with skin grafting, local or free flap transfers but recurred within 1 year post-operatively. The adipose-derived stem cells in culture demonstrate high and consistent cell proliferation, saturation density and colony-formign capacity. Additionally, the cells are highly inducible in differentiation to the adipose cells by a real-time BODIPYassay.
In a ShcC deficient mouse model, the artificial dermis alone failed to heal by 6 days post-operatively and the artificial wound healing in ShcC-deficient model with treatment of adipose-derived stem cells led to the almost equal wound healing with the control wild littermate animals.
The combined use of adipose-derived stem (regenerative) cells with the artificial dermis is effective in chronic wound healing such as radiation injuries and nerve-deficient conditions.
