抄録
Evaluation experiment of the Glutamyl cholin and V. B1 treatment was carried out using cynomolgus monkeys (body weight ca. 1.5kg). The viruses of poliomyelitis Lansing and Brunhilde strains were intracerebrally inoculated. When the infection was clinically confirmed by the onset of fever or paralysis, the treatment was immediately started. GLC 1cc 3mg mixed with V. B1 1cc 5mg was given daily by intrathecal injection.
The experiment for the Lansing strain challenged group was not successful. Five monkeys inoculated with the virus in dilution of 10-2 and 10-3 died and only one monkey survived. All three monkeys for the control died of the dsease with no exception.
Brunhilde strain was also challenged in the same way. The groups inoculated with the virus in dilution of 10-2 and 10-3 received the GLC and V. B1 treatment according to the above mentioned method. Three monkeys out of 9 (4monkeys are of 10-2 dilution group and 5 of 10-3 group) survived after the treatment.
Though it should be carefully refrained to conclude that the lower mortality rate in the treated group is statistically significant, close observation about the duration. and the clinical course of the disease implies a favorable prognosis of the disease, supporting the effectiveness of the treatment. When the harmonic means of the days elapsed from the onset of the clinical symptoms to the death of the animal were compared between the control and the treatment groups a noticeable prolongation of the days are recognized among the latter group. Also studying the fever curve and the course of the disease some monkeys of which the symptoms were all serious with the critical fall of body temperature below 35°C were found recovering (though paralysis remained) in the course of the treatment which lasted more than one month. So far in our experiment, monkeys intracerebrally challenged with poliomyelitis viruses and had the body temperature fallen below 35°C died unexceptionally when without GLC and V. B1 treatment.
All the observations from the various angles indicate that the treatment may be recommended as effective when it is adequately started from the early stage of the disease.
