ON A DOUBLE LYSOGENIC STRAIN OF Escherichia coli
1955 Volume 5 Issue 2 Pages 108-111
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1955 Volume 5 Issue 2 Pages 108-111
In the previous paper some lysogenic strains were uncovered among a number of Escherichia coli strains tested.
One of them was called A34 from which two kinds of phages were produced into the culture media following to the multiplication of bacteria. One phage called β was able to infect a strain of Shigella flexineri type 2, 2a, the other phage γ infect a strain of Shigella flexineri type 5.
After treating the broth culture at 58°C for 30 minutes, it was estimated to produce β phage to a titre of 106 per cc, whereas the titre of γ phage was only to about 102 per cc. It was shown by the neutralisation tests that these phages are serologically unrelated to each other.
In order to obtain the progeny of single cell, the technique of micromanipulation was used. According to these results, it was concluded that the capacity of producing both kinds of phages was transmitted from cell to cell indefinitely.
