インフルエンザウイルスの一定量法としてのLi-Rivers培養法の意義について 第1報

他の方法との比較について

抄録

The author made some discussion and experiment on the method of biological assay of virus, assuming that the individual sensitivity of experimental animal is normally distributed against the logarithmic dosis of infective viral agent.
A normal regression line, which was estimated most likely from the values of experimental probits of the ratio of reaction at various doses of virus, was, adopted as an approximation of the linear relationship between the logarithmic dosis of virus and the value of probit of corresponding population. A method was proposed to test the significance of the difference of gradients - i.e. square root of the reciprocal of the variance of sensitivity - of the two experimental normal regression lines which were obtained from the two series of experiments. This method was applied to the estimation of the quantity of the influenza virus, WS strain, by infecting mice, fetile hen's eggs and Li-Rivers' procedure. And the last method showed the smallest value of variance of sensitivity.
It was concluded that the cultivation method of Li and Rivers is, especially because of its higher reproducibility, equally or more useful than mice and fertile hen's eggs for the determination of fifty per cent effective endpoint of influenza virus.