In the neutralization experiments of a polyvalent temperate phage. β strain, using its specific anti rabbit serum, there observed two different neutralization curves by using two different indicator strains. That is, the valence for Shigella flexineri type 5 (Komagome A strain) was neutralized at a faster rate than the valence for Sh. flexineri Variant Y (Komagome BI strain). Neutralization velocity constant of β phage assayed on Komagome BI strain was about 60-80 min.-1, whereas that on Komagome A strain was about 300 min-1.
Several kinds of experiments were designed for its reason and it was concluded that this phenomenon due to the heterogeneous antigenic structure of each β phage particles.