Thin sections of the epithelial layer of rabbit cornea were needed for the electron microscopy of the intracellular form of vaccinia virus and herpes simplex virus multiplying in the infected rabbit cornea.
It was very hard to obtain consistently the thin sections of the epithelial layer only of rabbit cornea by using the usual method in which all the cornea sliced into small pieces were embedded into the embedding material and then cut.
It was found that it was very easy to obtain the thin sections of the epithelial layer only, if it was stripped from the proper layer of cornea prior to embedding into the methacrylate monomer.