BIOCHEMICAL STUDIES ON THE REPRODUCTION OF BACTERIOPHAGE

(I) STUDIES ON THE INITIAL REACTION IN PURINE-REQUIRING MUTANT OF E. COLI INFECTED WITH T2 PHAGE

Abstract

In delayed addition experiments with a purine-requiring mutant E. coli A-20, it was shown that an external source of purine was not required to support the early stages of phage development until 5 minutes after infection, provided glucose was added to the purine free medium. In this case, both RNA and protein can be formed immediately after infection in purine free medium, while uninfected bacteria can synthesize neither protein nor nucleic acids in this condition.