STUDIES OF CANARY POX VIRUS

2. VIRUS IN TISSUE CULTURE

Abstract

Canary pox virus was grown in cell culture of chick embryo.
1) The virus proliferated in the cultured cells with cytopathogenic effect. Serial passages were readily accomplished by inoculating infected culture fluid into fresh cultures.
2) The specific complement fixing antigen was found in infected culture fluid.
3) The characteristic cytoplasmic inclusions were formed in the infected cells.
4) A marked decrease of pH of the medium was noticed in the cell cultures showing marked the cytopathic changes.
5) The virus passaged in the cell culture produced the disease when inoculated to canaries.
6) The cell culture was successfully employed for primary isolation of the virus from naturally infected canaries.