STUDIES ON THE PHAGE-INDUCED INITIAL REACTIONS IN E. COLI
1. CHEMICAL FRACTIONATION OF “EARLY PROTEIN” SYNTHESIZED BY E. COLI INFECTED WITH BACTERIOPHAGE T2
1961 Volume 11 Issue 4 Pages 300-307
Details
1961 Volume 11 Issue 4 Pages 300-307
1) Crude extracts were prepared from T2-infected E. coli which incorporated S35O4--for a few minutes during the initial reaction period. The extracts were fractionated by starch zone electrophoresis, with ammonium sulphate, and by ultracentrifugation. Distributions of protein-S35 (“early protein”) among several fractions were studied and compared with that of protein-S35 (“coli type protein”) from crude extract of uninfected bacteria which were similarly treated. No difference of distributions has been observed with respect to the distribution of protein-S35 between the early protein and coli type protein.
2) Heavily ultraviolet light-irradiated bacteria lost the ability to assimilate S35 into proteins, while such bacteria infected with T2 phage assimilated S35 actively because of reactivation. Analysis of the early protein-S35 In T2-reactivated bacteria exhibited identical distribution of S35 as compared to distribution of S35 assimilated by uninfected bacteria.
3) In the identical condition of fractionation, striking abnormalities with respect to distribution of early RNA were observed as compared to that of coli RNA-distribution.
