CHROMATOGRAPHY OF TOBACCO MOSAIC VIRUS BY A CATION EXCHANGE RESIN

Abstract

A method is cescribed which shows that impurities contained in the chemically purified TMV preparations could be eliminated and two infective components could be separated by the use of strong cation exchange resin column (Amberlite IR-120).
The column (1×12cm) of the resin (Na-form, about 100 mesh size) was equilibrated with pH 5.0-5.2, M/15 phosphate buffer or M/25 citrate buffer solutions. Four to 8ml of TMV dissolved in the same buffer was added on the column. The column was then washed with 32ml of the same pH buffer, and 40ml of pH 6.2-6.3 and 40ml of pH 8.0-8.5 buffers were passed through the column. The impurities were contained in pH 5.0-5.2 fractions, and one infective component was obtained in pH 6.2-6.3 and the other in pH 8.0-8.5 fractions. The amount of the former component was much larger than that of the later.