Abstract
In this paper, an immunohistochemical study on the tumor antigen (T) induced by adenovirus type 12 infection to hamster embryo cells (HaE) in vitro was explored by the use of immunofluorescence technique. The infected cells were subjected to observe any antigenic alteration of T antigen stained with fluorescein isothiocyanate conjugated anti-T antigen hamster antibody after treatment of those cells with the following various reagents.
The antigenicity of T antigen was somewhat deteriorated by treatment with both proteolytic and carbohydrate-digesting enzymes and completely abolished with sodium periodate, phenol, deoxycholate, and sulfuric acid. However, it resisted to treatment with formaldehyde and organic solvents such as ethyl-ether, carbontetrachloride chloroform, while some hydrophilic organic solvents, for instance, acetone, methyl- and ethylalcohol acted somewhat deterioratively.
Interestingly, preabsorption of the conjugate with lactose resulted in inhibiting the positive fluorescence in T antigen
From these results, an assumption was made that adeno-12 induced T antigen in HaE would mainly consist of a protein which contains a part of polysuccharide like lactose as an immunodeterminant group.
