Abstract
Three strains of rubella virus could be isolated with primary African green monkey kidney (GMK) cell from the throat swab specimens of rubella patients during the epidemic at Osaka district in the spring of 1966. One of these newly isolated strains has been serially cultivated for at least 11 passages in the amniotic cavity of a developing chick embryo.
The chicken egg adapted strain was rather sensitive to temperatures as compared with other strain which was passaged only in GMK cell several times after isolation.
It was experimentally inoculated into cynomolgus monkeys at the 6th and 11th passage level and both produced sufficiently high NT (neutralization) and HI (hemagglutination-inhibition) antibody responses without any recognizable symptoms, although there were a little virus recoveries from the throat of some monkeys at both passage levels.
