Abstract
Crude or concentrated interferon (IF) solution from mouse brains infected with Japanese encephalitis virus (JEV) was administrated into mice before or after virus inoculation.
LD50's in the mice which were intraperitoneally pretreated with IF, then intraperitoneally infected with vesicular stomatitis virus (VSV), JEV and mouse hepatitis virus (MHV), were decreased 30-300 foldly compared with those in the control mice which had taken no IF pretreatment.
In the gronp of mice which had been infected with MHV (5LD50/mouse) and to which IF administration was begun at 4hrs post infection, mortality was reduced to 50% whereas all mice died in the covtrol group to which no IF was administrated.
As an in vitro model experiment for therapy against viral infection; onto the L cell monosheets on coverslips which had been infected with VSV (m.o.i. <<1), was added IF containing maintenance medium. And the process of inhibition of virus spreading to neighbouring cells and of “curing” of the monosheets from CPE was traced by staining with anti-VSV fluorescent antibody.
