Abstract
Studies were made on the physico-chemical properties of leucocytic pyrogens induced by influenza virus (V) and bacterial pyrogen of Escherichia coli (LPS) in rabbits. The following results were obtained.
1. The fever curve of the virus (256HAU/kg, i.v.) was quite different in lag time from that of LPS (0.1μg/kg, i.v.). The fever curves of the leucocytic pyrogens were quite similar to each other.
2. The chromatographic patterns over Sephadex (G-200) or Bio gel (P-150) of the two leucocytic pyrogens were shown to be the same as that of control leucocytic pyrogen.
3. The immuno-diffusion patterns of both leucocytic pyrogens against antiserum of guinea pig were the same, showing two or three precipitating bands.
From the results mentioned above, it was concluted that both pyrogens (V and LPS) might be mediated by the same leucocytic pyrogens.
