Abstract
Several mouse hybridoma cell lines producing antibodies against measles virus and HBs antigen were made by fusion of spleen cells, which were obtained from BALB/c mice immunized with these antigens, with P3X63Ag8 myeloma cells. The production of antibodies was screened by a radioimmunoassay using antigenprecoated polystyrene balls as a solid phase.
Twenty three hybridomas producing anti-measles antibody and 5 anti-HBs antibody were obtained as ascites form after intraperitoneal inoculation of hybridomas.
Out of 23 hybridomas producing antibodies against measles virus which showed very high but different values between HI and ELI antibody titers, 10 were producing antibodies against 79k measles hemagglutinin polypeptide. Cell membrane and cytoplasma of human B cell line persistently infected with measles virus (NC-S) showed a specific fluorescence when stained by hybridoma ascites and FITC labelled anti-mouse sera.
Hybridoma ascites producing anti-HBs antibody showed PHA titers as high as 400-320, 000fold. By Sephadex G-200 filtration and Ouchterlony technique it was found that antibodies from 4 hybridomas belonged to IgG class and those from one to IgM class. Subtype of HBs antigen was determined by R-PHA using sera of which subtypes of HBs antigen were already known and sheep erythrocytes coated by 3 hybridoma ascites, 2 of which were anti-r and one was anti-common determinant(s) including subtype a.
The present study suggests that these specific antibodies made by hybridoma technique will be useful for determining the localization of both measles virus and HBs antigen on the pathological materials obtained by biopsy and autopsy.
