Abstract
It is reported that mephenesinpropyleneglycol with tetracycline is clinically useful for the treatment of Japanese encephalitis. So we studied on virucidal effect of mephenesin against Japanese encephalitis virus by mice-inoculation test. Challenge to mice with Japanese encephalitis virus was tried with intraperitoneal inoculation and the mice were observed for 14 days after virus challenge.
10-1 of Japanese encephalitis virus infected mouse brain and mephenesinpropyleneglycol was injected intraperitoneally into mice. Mortality of mice injected with 1.25mg. of mephenesin was beneath of 60%, but that of mice injected with 0.62mg. of mephenesin was 80%.
Mice inoculated with Japanese encephalitis virus were injected with 0.125mg. of mephenesin at 1, 6, 12, 24, 48 or 72 hours after virus challenge, but mortality of the mice was about 80%. 1, 7, 13, 19, 25, 36, 48 and 72 hours after challenge with Japanese encephalitis virus, mice were injected with 0.025mg. or 0.012mg. of mephenesin at each time, but mortality of the mice were high.
In the similar experiments with mephenesin and chloramphenicol or with mephenesin and tetracycline, we did not recognize virucidal effect of mephenesin against Japanese encephalitis virus.
Our experiments show that the drug has weak ability of inactivation of Japanese encephalitis virus only in vitro, but that in vivo has no effect.
