Abstract
It has been found that the intradermal inoculation into rabbits of influenza virus (PR 8) suspensions caused edema, erythema and sometimes slight subcutaneous hemorrhage. Microscopically, infiltration of leucocytes, dilatation of blood capillaries, hemorrhage and edema in corium and degeneration of the epithelial cells were observed.
Hemagglutination titer in the site of the inoculation was decrease rapidly within 24 hours after inoculation, and multiplication of the virus could not be detected at the site.
Active immunization could not inhibit completely the production of skin lesion, but skin lesion-producing effect of the virus was neutralized by antiviral serum.
Skin lesion-producing effect of the virus was destroyed markedly by inactivation of the virus with merzonin, heat or ultraviolet irradiation, and it was strengthened by sonic vibration and by repeated freezing and thowing. These features were similar to the results obtained in lung consolidation-producing effect and lethal effect of the virus in mice.
Some autonomic blocking agents and corticosteroid hormone had almost no effect on the production of skin lesion by the virus except tetraethylammonium bromide had slightly inhibitory effect.
