Abstract
Acetylcholine sensor based on ISFET as a pH sensor, using the immobilized acetylcholine esterase (AChE) in the BSA membrane are reported for the characteristics and for measuring chemicals. We attempted the application of an enzyme sensor based on ISFET as an effective and simple potentiometric sensor for the detection of chemicals, as irreversible and reversible inhibitors. New data on the analysis of chemicals are reported using the present acetylcholine sensor based on the inhibition of the immobilized AChE. Reactivation of immobilized AChE after inhibition with reversible and irreversible inhibitors, was carried out using a mixture of acetylcholine and pyridine-2-aldoxime methiodide (PAM). The detection for PHC and Nicotine as reversible inhibitors, and Malathion and Dimethoate as irreversible inhibitors was found to be in the ranges of 5×10-5-5×10-4 M, 10-5-10-4 M, 10-6-10-4 and 2.5×10-5-10-3 M, respectively.
The present study was also developed for an electrochemical sensor for toxic substances which works by measuring the inhibition of the enzyme activity. We suggested that these inhibition rates (e.g., I50) by the biosensor may be important as a biological index to biological systems with regard to toxic matters.
