Abstract
According to the appointment of Ashby et al., 60% of the environmental carcinogens are negative in Ames test. About non-mutagenic carcinogens, it is reported that there is the relationship between carcinogenicity and the ability to produce active oxygen.
We choosed organochlorine compounds which are non-mutagenic carcinogens and nitroarenes as test chemicals.
As a means to evaluate the ability of chemicals to produce O2-, new strain which can detect soxS induced by O2- is used. And for positive compounds, umu test is tried. And whether the test with the new strain can become a way of screeing of non-mutagenic carcinogens is discussed.
As a result of these tests, it is cleared that there are compounds which produce O2- and H2O2 induced by the O2- causes DNA damage. This result suggests that new strain can become a means to screen non-mutagenic carcinogens. Intensity of mutagenic activity of organochlorine compounds in umu test decreased when S9mix was added. When 1-naphthol and quinoline are nitrated, they become to produce O2-. Aromatic compounds existing in tar can get ability to produce O2- after nitrated and can cause DNA damage. Therefore the evaluation of toxic effects of active oxygen should be added to established tests.
