According to the appointment of Ashby et al., 60% of the environmental carcinogens are negative in Ames test. About non-mutagenic carcinogens, it is reported that there is the relationship between carcinogenicity and the ability to produce active oxygen.
We choosed organochlorine compounds which are non-mutagenic carcinogens and nitroarenes as test chemicals.
As a means to evaluate the ability of chemicals to produce O
2-, new strain which can detect
soxS induced by O
2- is used. And for positive compounds,
umu test is tried. And whether the test with the new strain can become a way of screeing of non-mutagenic carcinogens is discussed.
As a result of these tests, it is cleared that there are compounds which produce O
2- and H
2O
2 induced by the O
2- causes DNA damage. This result suggests that new strain can become a means to screen non-mutagenic carcinogens. Intensity of mutagenic activity of organochlorine compounds in
umu test decreased when S9mix was added. When 1-naphthol and quinoline are nitrated, they become to produce O
2-. Aromatic compounds existing in tar can get ability to produce O
2- after nitrated and can cause DNA damage. Therefore the evaluation of toxic effects of active oxygen should be added to established tests.
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