Abstract
In the previous works, we performed experimental evaluations of cytotoxicity of the Tama-river water and found that the daily cytotoxicity variation of this river water was so large that the water may cause serious effects on human body in a short period of time. From these findings, we confirmed that the rapid and simple bioassay for detecting toxicity should be developed in very near future. As a rapid detection method, LDL (low density lipoprotein)-uptaking activity of mammalian cell is considered to be one of the most rapid endpoints for the cells exposed to toxicic substances. In this work, a novel bioassay based on LDL-uptaking activity was proposed and developed, which successfully detected rive water cytotoxicity very quickly as compared with other methods using cultured cell. The methodology developed in this work is considered to be one of the promising canditates of bioassay sensors for water quality management.
