Journal of Japan Society on Water Environment
Online ISSN : 1881-3690
Print ISSN : 0916-8958
ISSN-L : 0916-8958
ORIGINALS
Toxicity Assessment of Organochlorine Compounds Detected in Water Environment Using Cultured Human Cell Lines
Manabu KUNIMOTOJunzo YONEMOTOYuko SOMAOsami NAKASUGI
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1997 Volume 20 Issue 11 Pages 752-756

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Abstract
As part of validation processes of in vitro toxicity assays for the risk assessment of environmental hazards, we applied an in vitro toxicity test using two human cell lines, neuroblastoma NB-1 cells and glioblastoma U-87 MG cells, to the assessment of organochlorine compounds detected in the water environment. The in vitro toxicity assay using NB-1 cells was calibrated by testing reference chemicals proposed by MEIC (Multicenter Evaluation of In Vitro Cytotoxicity), an international program for the validation of in vitro cytotoxicity assays.
Beforehand, an assay using cells in frozen stock without subcultivation was examined by comparing IC50 values with the ordinary assay using subcultured cells. IC50 values for MEIC reference chemicals from the former assay showed good correlation with those from the latter assay, suggesting that the assay using cells in frozen stock can he used at least for the assessment of basal cytotoxicity.
IC50 values for ten organochlorine compounds frequently detected in the sediment samples from contaminated rivers, p-chloroaniline, 3,4-dichloroaniline, p-dichlorohenzene, o-dichlorobenzene, Tris (2-chloroethyl)-phosphate, 2,5-dichlorophenol, 2,5-dichloroanisol, Triclosan and Triclocarban, were obtained with the in vitro assays and compared with their LD50 values in rats. No significant correlation, however, was seen between the IC50 and LD50 values, indicating that further improvement of in vitro toxicity assays is necessary for the application to the risk assessment of environmental hazards.
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© 1997 Japan Society on Water Environment
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