Abstract
Four monoclonal antibodies (MAb-No.1, 2, 3 and 4) were raised against a conjugate of camphor linked to bovine serum albumin. These antibodies showed affinity to 2-methylisoborneol (2-MIB), a common cause for earthy/musty off-flavor. The sensitivity and specificity of the antibodies were tested by ELISA in a microtiter plate coated with a camphor-ovalbumin conjugate. Specificity of these antibodies were tested by using camphor, camphorquinone, norcamphor, 2-methylisoborneol, borneol, isoborneol, norbornane and norborneol. Although MAb-No.2 and 3 were reactive against all of the compounds tested, MAb-No.1 and 4 did not react with norcamphor, norbornane and norborneol. MAb-No.4 was purified from a culture supernatant. 2-MIB was detected even at 1 μg·ml-1 by the ELISA using the purified MAb-No.4 at 0.63μg·ml-1.
